EFÈ ANTI-INFLAMMATOIRES PERILLA FRUTESCENS FÈY RALE SOU STIMULER LIPOPOLYSACHARIDE KAW264.7CELL

Efè anti-inflammatoires Perilla retire de stimuler Lipolysacharide RAW264.7cell


Perilla fèy yo lajman te itilize nan remèd fèy Chinwa yo ak nan Japonè e ajan yo te itilize pou trete rale lè maladi. Etid sa a ki te ame li pou

envestige efè anti‑inflammatory ak de fòmil de Perilla frutescens fèy retire (re Tir). Murine macrophage RAW264.7 cellules

te itilize nan kòm yon modèl. Selil viabilité ak chanjman morphologiques te etidye lang sa pa Devront essai e microscopie. mRNA ekspresyon de

pro‑inflammatory medyatè te évalué pa semi‑quantitative transcription‑polymerase lanvè chèn reyaksyon (RT‑PCR) ak quantitative (q)

RT‑PCR. Ksid nitrique (pa) ak prostaglandin E2 (PGE2) pwodiksyon te analysé pa a Griess tès ak sandwich enzyme‑linked immunosorbent

essai (ELISA), respektivman. Activation kinase cascades ki te etidye lang pa immunoblotting. Jwenn nou te montre sa ki yon ti jan anpil sèlil

viabilité, men limite activation LPS‑induced de cellules RAW264.7. Epitou, re Tir siyifikativman te redwi a ekspresyon LPS‑induced mRNA de la

interleukin (IL) ‑6, IL‑8, timè nécrose factor‑α (TNF‑α), cyclooxygenase‑2 (COX‑2) ak synthase ksid nitrique inducible (iNOS), gènes nan yon

dose‑dependent fason. An plis, ki te redwi pa pwodiksyon ak sòs PGE2 par pa LPS. Re Tir tou jennen activation de

mitogen‑activated pwoteyin kinases (MAPKs), te ogmante nivo IκBα cytosolic, e te redwi nivo ‑κB nikleyè faktè (NF). Pran ansanm, sa

jwenn endike ke re Tir siyifikativman diminue mRNA espresyon ak pwoteyin pwodiksyon medyatè pro‑inflammatory, via anpèchman de

kinase extracellular‑Signal‑regulated (ERK) 1/2, c‑Jun N‑terminal kinase (JNK), p38, tankou NF‑κB ap sonnen de sinyalman nan RAW264.7 cellules stimuler ak LPS.